Thomas Appleton Esq. was born about 1538 in Little Waldingfield, co. Suffolk, England. He signed a will on 1 Mar 1603. He died between 1 Mar 1603 and 16 May 1603. His estate was probated on 16 May 1603. He was married to Mary Isaac about 1568.

Mary Isaac was born about 1550. She was imprisoned in the Fleet in 1604. She was "enlarged of her imprisonment out of the Fleet where she remaineth" on 18 Dec 1604. She died on 18 Feb 1612/13. Her estate was probated on 11 Jun 1613. Children were:

i. Judith Appleton was born about 1570. She died in infancy.
ii. Mary Appleton was born about 1572. She died before 1637.
iii. Judith Appleton was baptized on 10 Oct 1574 in Little Waldingfield, co. Suffolk, England.
iv. Sir Isaac Appleton was born about 1576. He was buried on 14 Jun 1615 in Little Waldingfield, co. Suffolk, England.
v. Sarah Appleton was born in 1580.
vi. John Appleton was baptized in 1582. He signed a will on 8 Jan 1622. His estate was probated on 17 May 1631.
vii. Thomas Appleton died after 1603.
viii. Samuel Appleton.