William Twining Sr. was born about 1600 in England. He died on 15 Apr 1659 in Eastham, Barnstable Co., Massachusetts. He was married to Ann about 1621.

Ann was born about 1605. She died on 27 Feb 1680 in Eastham, Barnstable Co., Massachusetts. Children were:

i. Isabel Twining.
ii. William Twining Jr..