Ephraim Doane was born before 1645 in Plymouth, Plymouth Co., Massachusetts. He took the oath of fidelity in 1670. He took the oath of freeman on 5 Jun 1684. He was a surveyor of highways from 1691 to 1692 in Eastham, Barnstable Co., Massachusetts. He signed a will on 17 Dec 1699. He died in 1700 in Eastham, Barnstable Co., Massachusetts. His estate was probated on 18 Apr 1700. He was married to Mercy Knowles on 5 Feb 1667/68 in Eastham, Barnstable Co., Massachusetts.

Mercy Knowles was born in Plymouth, Plymouth Co., Massachusetts. She was also known as Mary Knowles. Children were:

i. Patience Doane was born on 28 Jan 1668/69 in Eastham, Barnstable Co., Massachusetts. She died in 1675.
ii. Apphia Doane was born on 18 Jul 1670 in Eastham, Barnstable Co., Massachusetts.
iii. Hezekiah Doane was born in Aug 1672 in Eastham, Barnstable Co., Massachusetts. He died in 1752 in Wellfleet, Barnstable Co., Massachusetts. He was a whale fisherman. He married Hannah Snow about 1691 in Eastham, Barnstable Co., Massachusetts.
iv. Thomas Doane.
v. Ebenezer Doane was born in Apr 1676 in Eastham, Barnstable Co., Massachusetts.
vi. Nehemiah Doane was born in Aug 1680 in Eastham, Barnstable Co., Massachusetts. He died in Feb 1684.
vii. Patience Doane was born in Apr 1682 in Eastham, Barnstable Co., Massachusetts.
viii. Ruhama Doane was born on 30 Apr 1685 in Eastham, Barnstable Co., Massachusetts.

Ephraim Doane married second Mary Smalley after 20 Apr 1692.